{"id":1552,"date":"2015-02-01T00:00:02","date_gmt":"2015-02-01T00:00:02","guid":{"rendered":"http:\/\/bergleslab.com\/wordpress\/?p=1552"},"modified":"2017-10-18T19:29:16","modified_gmt":"2017-10-18T19:29:16","slug":"otsu2015","status":"publish","type":"post","link":"http:\/\/bergleslab.com\/index.php\/2015\/02\/01\/otsu2015\/","title":{"rendered":"Calcium dynamics in astrocyte processes during neurovascular coupling."},"content":{"rendered":"
Nat Neurosci.<\/a> 2015 Feb;18(2):210-8. doi: 10.1038\/nn.3906. Epub 2014 Dec 22.<\/div>\n

Calcium dynamics in astrocyte processes during neurovascular coupling.<\/h4>\n
Otsu Y<\/a>1<\/sup>, Couchman K<\/a>1<\/sup>, Lyons DG<\/a>1<\/sup>, Collot M<\/a>2<\/sup>, Agarwal A<\/a>3<\/sup>, Mallet JM<\/a>2<\/sup>, Pfrieger FW<\/a>4<\/sup>, Bergles DE<\/a>3<\/sup>, Charpak S<\/a>1<\/sup>.<\/div>\n

<\/h4>\n

Abstract<\/h4>\n
\n

Enhanced neuronal activity in the brain triggers a local increase in blood flow, termed functional hyperemia, via several mechanisms, including calcium (Ca(2+)) signaling in astrocytes. However, recent in vivo studies have questioned the role of astrocytes in functional hyperemia because of the slow and sparse dynamics of their somatic Ca(2+) signals and the absence of glutamate metabotropic receptor 5 in adults. Here, we reexamined their role in neurovascular coupling by selectively expressing a genetically encoded Ca(2+) sensor in astrocytes of the olfactory bulb. We show that in anesthetized mice, the physiological activation of olfactory sensory neuron (OSN) terminals reliably triggers Ca(2+) increases in astrocyte processes but not in somata. These Ca(2+) increases systematically precede the onset of functional hyperemia by 1-2 s, reestablishing astrocytes as potential regulators of neurovascular coupling.<\/p>\n<\/div>\n

 <\/p>\n

1 [1] Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1128, Paris, France. [2] Laboratory of Neurophysiology and New Microscopies, Universit\u00e9 Paris Descartes, Paris, France.<\/p>\n

2 [1] Centre National de la Recherche Scientifique (CNRS), UMR 7203, Paris, France. [2] Laboratory of Biomolecules, Universit\u00e9 Pierre et Marie Curie, Paris, France.<\/p>\n

3 The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.<\/p>\n

4 CNRS UPR 3212, University of Strasbourg, Institute of Cellular and Integrative Neurosciences (INCI), Strasbourg, France.<\/p>\n","protected":false},"excerpt":{"rendered":"

Enhanced neuronal activity in the brain triggers a local increase in blood flow, termed functional hyperemia, via several mechanisms, including calcium (Ca(2+)) signaling in astrocytes. However, recent in vivo studies have questioned the role of astrocytes in functional hyperemia because of the slow and sparse dynamics of their somatic Ca(2+) signals and the absence of glutamate metabotropic receptor 5 in adults. Here, we reexamined their role in neurovascular coupling by selectively expressing a genetically encoded Ca(2+) sensor in astrocytes of the olfactory bulb. We show that in anesthetized mice, the physiological activation of olfactory sensory neuron (OSN) terminals reliably triggers Ca(2+) increases in astrocyte processes but not in somata. These Ca(2+) increases systematically precede the onset of functional hyperemia by 1-2 s, reestablishing astrocytes as potential regulators of neurovascular coupling.<\/p>\n","protected":false},"author":1,"featured_media":1897,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[78,33],"tags":[29,30,39],"class_list":["post-1552","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-original-research","category-publications","tag-astrocytes","tag-calcium","tag-neurovascular-coupling"],"_links":{"self":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts\/1552","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/comments?post=1552"}],"version-history":[{"count":2,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts\/1552\/revisions"}],"predecessor-version":[{"id":2091,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts\/1552\/revisions\/2091"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/media\/1897"}],"wp:attachment":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/media?parent=1552"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/categories?post=1552"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/tags?post=1552"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}