{"id":1559,"date":"2014-09-01T00:00:41","date_gmt":"2014-09-01T00:00:41","guid":{"rendered":"http:\/\/bergleslab.com\/wordpress\/?p=1559"},"modified":"2017-10-18T19:30:09","modified_gmt":"2017-10-18T19:30:09","slug":"baxi2014","status":"publish","type":"post","link":"http:\/\/bergleslab.com\/index.php\/2014\/09\/01\/baxi2014\/","title":{"rendered":"A selective thyroid hormone \u03b2 receptor agonist enhances human and rodent oligodendrocyte differentiation."},"content":{"rendered":"
Glia.<\/a> 2014 Sep;62(9):1513-29. doi: 10.1002\/glia.22697. Epub 2014 May 24.<\/div>\n

A selective thyroid hormone \u03b2 receptor agonist enhances human and rodent oligodendrocyte differentiation.<\/h4>\n
Baxi EG<\/a>, Schott JT<\/a>, Fairchild AN<\/a>, Kirby LA<\/a>, Karani R<\/a>, Uapinyoying P<\/a>, Pardo-Villamizar C<\/a>, Rothstein JR<\/a>, Bergles DE<\/a>, Calabresi PA<\/a>.<\/div>\n

<\/h4>\n

Abstract<\/h4>\n
\n

Nerve conduction within the mammalian central nervous system is made efficient by oligodendrocyte-derived myelin. Historically, thyroid hormones have a well described role in regulating oligodendrocyte differentiation and myelination during development; however, it remains unclear which thyroid hormone receptors are required to drive these effects. This is a question with clinical relevance since nonspecific thyroid receptor stimulation can produce deleterious side-effects. Here we report that GC-1, a thyromimetic with selective thyroid receptor \u03b2 action and a potentially limited side-effect profile, promotes in vitro oligodendrogenesis from both rodent and human oligodendrocyte progenitor cells. In addition, we used in vivo genetic fate tracing of oligodendrocyte progenitor cells via PDGF\u03b1R-CreER;Rosa26-eYFP double-transgenic mice to examine the effect of GC-1 on cellular fate and find that treatment with GC-1 during developmental myelination promotes oligodendrogenesis within the corpus callosum, occipital cortex and optic nerve. GC-1 was also observed to enhance the expression of the myelin proteins MBP, CNP and MAG within the same regions. These results indicate that a \u03b2 receptor selective thyromimetic can enhance oligodendrocyte differentiation in vitro and during developmental myelination in vivo and warrants further study as a therapeutic agent for demyelinating models.<\/p>\n<\/div>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Nerve conduction within the mammalian central nervous system is made efficient by oligodendrocyte-derived myelin. Historically, thyroid hormones have a well described role in regulating oligodendrocyte differentiation and myelination during development; however, it remains unclear which thyroid hormone receptors are required to drive these effects. This is a question with clinical relevance since nonspecific thyroid receptor stimulation can produce deleterious side-effects. Here we report that GC-1, a thyromimetic with selective thyroid receptor \u03b2 action and a potentially limited side-effect profile, promotes in vitro oligodendrogenesis from both rodent and human oligodendrocyte progenitor cells. In addition, we used in vivo genetic fate tracing of oligodendrocyte progenitor cells via PDGF\u03b1R-CreER;Rosa26-eYFP double-transgenic mice to examine the effect of GC-1 on cellular fate and find that treatment with GC-1 during developmental myelination promotes oligodendrogenesis within the corpus callosum, occipital cortex and optic nerve. GC-1 was also observed to enhance the expression of the myelin proteins MBP, CNP and MAG within the same regions. These results indicate that a \u03b2 receptor selective thyromimetic can enhance oligodendrocyte differentiation in vitro and during developmental myelination in vivo and warrants further study as a therapeutic agent for demyelinating models.<\/p>\n","protected":false},"author":1,"featured_media":1905,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[78,33],"tags":[48,74,73],"class_list":["post-1559","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-original-research","category-publications","tag-oligodendrocyte-precursor-cells","tag-oligodendrogenesis","tag-thyroid-hormones"],"_links":{"self":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts\/1559","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/comments?post=1559"}],"version-history":[{"count":2,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts\/1559\/revisions"}],"predecessor-version":[{"id":2094,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts\/1559\/revisions\/2094"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/media\/1905"}],"wp:attachment":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/media?parent=1559"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/categories?post=1559"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/tags?post=1559"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}