{"id":1573,"date":"2013-06-01T00:00:31","date_gmt":"2013-06-01T00:00:31","guid":{"rendered":"http:\/\/bergleslab.com\/wordpress\/?p=1573"},"modified":"2017-10-18T19:33:54","modified_gmt":"2017-10-18T19:33:54","slug":"hughes2013","status":"publish","type":"post","link":"http:\/\/bergleslab.com\/index.php\/2013\/06\/01\/hughes2013\/","title":{"rendered":"Oligodendrocyte progenitors balance growth with self-repulsion to achieve homeostasis in the adult brain."},"content":{"rendered":"
Nat Neurosci.<\/a> 2013 Jun;16(6):668-76. doi: 10.1038\/nn.3390. Epub 2013 Apr 28.<\/div>\n

Oligodendrocyte progenitors balance growth with self-repulsion to achieve homeostasis in the adult brain.<\/h4>\n
Hughes EG<\/a>, Kang SH<\/a>, Fukaya M<\/a>, Bergles DE<\/a>.<\/div>\n

<\/h4>\n

Abstract<\/h4>\n
\n

The adult CNS contains an abundant population of oligodendrocyte precursor cells (NG2(+) cells) that generate oligodendrocytes and repair myelin, but how these ubiquitous progenitors maintain their density is unknown. We generated NG2-mEGFP mice and used in vivo two-photon imaging to study their dynamics in the adult brain. Time-lapse imaging revealed that NG2(+) cells in the cortex were highly dynamic; they surveyed their local environment with motile filopodia, extended growth cones and continuously migrated. They maintained unique territories though self-avoidance, and NG2(+) cell loss though death, differentiation or ablation triggered rapid migration and proliferation of adjacent cells to restore their density. NG2(+) cells recruited to sites of focal CNS injury were similarly replaced by a proliferative burst surrounding the injury site. Thus, homeostatic control of NG2(+) cell density through a balance of active growth and self-repulsion ensures that these progenitors are available to replace oligodendrocytes and participate in tissue repair.<\/p>\n<\/div>\n

PDF<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

The adult CNS contains an abundant population of oligodendrocyte precursor cells (NG2(+) cells) that generate oligodendrocytes and repair myelin, but how these ubiquitous progenitors maintain their density is unknown. We generated NG2-mEGFP mice and used in vivo two-photon imaging to study their dynamics in the adult brain. Time-lapse imaging revealed that NG2(+) cells in the cortex were highly dynamic; they surveyed their local environment with motile filopodia, extended growth cones and continuously migrated. They maintained unique territories though self-avoidance, and NG2(+) cell loss though death, differentiation or ablation triggered rapid migration and proliferation of adjacent cells to restore their density. NG2(+) cells recruited to sites of focal CNS injury were similarly replaced by a proliferative burst surrounding the injury site. Thus, homeostatic control of NG2(+) cell density through a balance of active growth and self-repulsion ensures that these progenitors are available to replace oligodendrocytes and participate in tissue repair.<\/p>\n","protected":false},"author":1,"featured_media":1907,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[78,33],"tags":[83,46,48],"class_list":["post-1573","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-original-research","category-publications","tag-homeostasis","tag-in-vivo-imaging","tag-oligodendrocyte-precursor-cells"],"_links":{"self":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts\/1573","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/comments?post=1573"}],"version-history":[{"count":3,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts\/1573\/revisions"}],"predecessor-version":[{"id":2102,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/posts\/1573\/revisions\/2102"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/media\/1907"}],"wp:attachment":[{"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/media?parent=1573"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/categories?post=1573"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/bergleslab.com\/index.php\/wp-json\/wp\/v2\/tags?post=1573"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}